SRX2478918: Castor canadensis Illumina WGS3
4 ILLUMINA (HiSeq X Five) runs: 991.8M spots, 299.5G bases, 110.1Gb downloads
Design: Whole-genome, short-read sequencing was done using the Illumina HiSeq X platform. Purified genomic DNA from fresh beaver blood leukocytes was quantified by Qubit DNA HS Assay (ThermoFisher). Genomic libraries were constructed using the Illumina Nano DNA Library Preparation Kit from DNA sheared using a Covaris LE220 Focused Ultrasonicator (Covaris, Woburn, MA, USA) to an average length between 300 and 400 bases. Sheared DNA (100ng) for library preparation followed Illumina’s recommendation, and six amplification cycles. Libraries were quantified for sample-loading using the KAPA Library Quantification Kit (Roche Diagnostics), producing multiple lanes of DNA sequences (150 base paired-end reads of a 300-400 base insert).
Submitted by: Hospital for Sick Children
Study:
Castor canadensis Genome - Transcriptome Sequencing and Assemblyshow Abstracthide AbstractCastor canadensis Genome sequencing and assembly
Sample:
Model organism - Castor canadensisLibrary:
Name: pair_end_raw_3
Instrument: HiSeq X Five
Strategy: WGS
Source: GENOMIC
Selection: RANDOM
Layout: PAIRED
Runs:
4 runs, 991.8M spots, 299.5G bases, 110.1Gb